The objective of this project is to develop and identify cassava genotypes whose roots produce amylose-free starch and are adapted to the environmental growing conditions in the northern coast of Colombia, and that express the Waxy gene discovered by CIAT in cassava genotype AM 206-5.
The project is being implemented in three steps:
Cross the source (AM 206-5) of the mutation with materials adapted to the target environment. The result of these crosses (F1) will be segregating germplasm that will not produce waxy starch (the homozygous condition in AM 206-5 will be lost) but combines the mutation with genes for adaptation to Brazilian conditions.
Make crosses among unrelated F1 genotypes to generate what could be considered as an F2 generation. This widely variable population will allow for the recovery of genotypes with amylose-free starch (25% of the F2 should have this characteristic). This approach will allow for the recovery of the waxy starch trait but without a major increase in the inbreeding coefficient (and therefore there will be negligible inbreeding depression).
Select the waxy starch genotypes and evaluate them under proper environmental conditions for adequate agronomic performance.